CD22 anticorps

N° du produit ABIN7651018

Cet anticorps Souris Monoclonal détecte spécifiquement CD22 dans FACS et Func. Il présente une réactivité envers Humain.

Aperçu rapide pour CD22 anticorps (ABIN7651018)

Antigène: CD22 (CD22 Molecule (CD22))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD22 est non-conjugé

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: RFB4

Détail du produit anti-CD22 anticorps

Fonction: CD22 / BL-CAM (B-Cell Marker)(RFB4)

Attributs du produit: Recognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD . It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Human tonsil lymphocytes.

Isotype: IgG1, kappa

Information d'application

Indications d'application: ELISA: For coating, order antibody without BSA, Flow cytometry: 0.5-1 μg/million cells, Immunofluorescence: 0.5-1 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Commentaires:

Positive Control: Ramos, Raji or Daudi cells. Human Tonsil, Lymph Node or Spleen.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur CD22

Antigène: CD22 (CD22 Molecule (CD22))

Autre désignation: BL-CAM

Sujet:

Synonyms: B-lymphocyte cell adhesion molecule (BL-CAM), B-cell receptor CD22, CD22, Lectin 2, Lyb8, Sialic acid-binding Ig-like lectin 2 (Siglec-2), SIGLEC2, T-cell surface antigen Leu-14

Gene Symbol: CD22

Tissue Expression: B-cells|Lymphocytes

Poids moléculaire: 130-140 kDa

ID gène: 933

UniProt: P20273